rna fluorescence in situ hybridization kit (Servicebio Inc)
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Rna Fluorescence In Situ Hybridization Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+in+situ+hybridization+kit/fixative+hybridization+situ/pmc13050104-98-9-15
Average 86 stars, based on 1 article reviews
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Fluorescence:Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927. Article Title: Single-nucleus rna sequencing identifies universal camk1d upregulation and dysregulated c-ltmr subtypes as key drivers of paclitaxel-induced neuropathy. Article Snippet: The snRNA libraries were sequenced on GenoLab M platform (GeneMind Biosciences, Shenzhen, China) via PE150 model (Pavel et al. 2023). .. The fluorescence in situ hybridization (FISH) FISH assay was performed using a Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B Article Snippet: .. Fluorescence in situ hybridization (FISH) The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in Article Title: ANRIL modulates endothelial senescence and angiogenesis through SASP-driven miR146a regulation in age-related vascular dysfunction. Article Snippet: Vascular aging, marked by endothelial cell (EC) dysfunction and compromised angiogenesis, is a central driver of age-related ischemic diseases.. Although lncRNAs have emerged as pivotal regulators of endothelial function, their specific roles in endothelial aging remain enigmatic.. In this study, we identify the lncRNA ANRIL as a crucial modulator of endothelial dysfunction during aging. In Situ Hybridization:Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927. Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via Article Title: Circ_0057105 promotes intrahepatic cholangiocarcinoma progression by sponging miR-1290 and regulating the MDM2/P53 pathway. Article Snippet: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive malignancy with limited treatment options, underscoring the need to identify new regulatory molecules.. Through high-throughput sequencing of five paired ICC and adjacent normal tissues, we identified a significantly upregulated circular RNA, circ_0057105, which is further validated in ICC cell lines and clinical samples.. A correlation between elevated circ_0057105 expression and poor prognosis in ICC patients was observed based on clinical data. Article Title: Single-nucleus rna sequencing identifies universal camk1d upregulation and dysregulated c-ltmr subtypes as key drivers of paclitaxel-induced neuropathy. Article Snippet: The snRNA libraries were sequenced on GenoLab M platform (GeneMind Biosciences, Shenzhen, China) via PE150 model (Pavel et al. 2023). .. The fluorescence in situ hybridization (FISH) FISH assay was performed using a Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B Article Snippet: .. The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in Article Title: LINC01116 modulates EMT process via binding with AGO1 mRNA in oesophageal squamous cell carcinoma. Article Snippet: For FISH assay, DIGlabeled LINC01116 probe was purchased by Servicebio Company (Wuhan, China). .. Hybridization was performed overnight at 37 ◦C using a fluorescent in Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B Article Snippet: .. Fluorescence in situ hybridization (FISH) The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in Article Title: ANRIL modulates endothelial senescence and angiogenesis through SASP-driven miR146a regulation in age-related vascular dysfunction. Article Snippet: Vascular aging, marked by endothelial cell (EC) dysfunction and compromised angiogenesis, is a central driver of age-related ischemic diseases.. Although lncRNAs have emerged as pivotal regulators of endothelial function, their specific roles in endothelial aging remain enigmatic.. In this study, we identify the lncRNA ANRIL as a crucial modulator of endothelial dysfunction during aging. Fluorescence In Situ Hybridization:Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927. Article Title: Single-nucleus rna sequencing identifies universal camk1d upregulation and dysregulated c-ltmr subtypes as key drivers of paclitaxel-induced neuropathy. Article Snippet: The snRNA libraries were sequenced on GenoLab M platform (GeneMind Biosciences, Shenzhen, China) via PE150 model (Pavel et al. 2023). .. The fluorescence in situ hybridization (FISH) FISH assay was performed using a Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B Article Snippet: .. The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B Article Snippet: .. Fluorescence in situ hybridization (FISH) The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in Article Title: ANRIL modulates endothelial senescence and angiogenesis through SASP-driven miR146a regulation in age-related vascular dysfunction. Article Snippet: Vascular aging, marked by endothelial cell (EC) dysfunction and compromised angiogenesis, is a central driver of age-related ischemic diseases.. Although lncRNAs have emerged as pivotal regulators of endothelial function, their specific roles in endothelial aging remain enigmatic.. In this study, we identify the lncRNA ANRIL as a crucial modulator of endothelial dysfunction during aging. Immunocytochemistry:Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927. Article Title: Circ_0057105 promotes intrahepatic cholangiocarcinoma progression by sponging miR-1290 and regulating the MDM2/P53 pathway. Article Snippet: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive malignancy with limited treatment options, underscoring the need to identify new regulatory molecules.. Through high-throughput sequencing of five paired ICC and adjacent normal tissues, we identified a significantly upregulated circular RNA, circ_0057105, which is further validated in ICC cell lines and clinical samples.. A correlation between elevated circ_0057105 expression and poor prognosis in ICC patients was observed based on clinical data. Hybridization:Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927. Article Title: Circ_0057105 promotes intrahepatic cholangiocarcinoma progression by sponging miR-1290 and regulating the MDM2/P53 pathway. Article Snippet: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive malignancy with limited treatment options, underscoring the need to identify new regulatory molecules.. Through high-throughput sequencing of five paired ICC and adjacent normal tissues, we identified a significantly upregulated circular RNA, circ_0057105, which is further validated in ICC cell lines and clinical samples.. A correlation between elevated circ_0057105 expression and poor prognosis in ICC patients was observed based on clinical data. Article Title: LINC01116 modulates EMT process via binding with AGO1 mRNA in oesophageal squamous cell carcinoma. Article Snippet: For FISH assay, DIGlabeled LINC01116 probe was purchased by Servicebio Company (Wuhan, China). .. Hybridization was performed overnight at 37 ◦C using a fluorescent in Imaging:Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via Microscopy:Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via Confocal Microscopy:Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via |

