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Servicebio Inc rna fluorescence in situ hybridization kit
Rna Fluorescence In Situ Hybridization Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+in+situ+hybridization+kit/fixative+hybridization+situ/pmc13050104-98-9-15
Average 86 stars, based on 1 article reviews
rna fluorescence in situ hybridization kit - by Bioz Stars, 2026-09
86/100 stars

Images

Related Articles

Fluorescence:

Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway
Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927.

Article Title: Single-nucleus rna sequencing identifies universal camk1d upregulation and dysregulated c-ltmr subtypes as key drivers of paclitaxel-induced neuropathy.
Article Snippet: The snRNA libraries were sequenced on GenoLab M platform (GeneMind Biosciences, Shenzhen, China) via PE150 model (Pavel et al. 2023). .. The fluorescence in situ hybridization (FISH) FISH assay was performed using a Fluorescent In Situ Hybridization Kit (Servicebio, Wuhan, China) following the manufacturer’s protocols. .. The hybridization utilized Camk1d (Camk1d probe sequence 5′- GGG TAA CCA CAG AGC AAG ATA TAG GCG3′) with Alexa Cy3-labeled and 488-labeled probes (Obio Technology, Shanghai, China), respectively.

Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B
Article Snippet: .. Fluorescence in situ hybridization (FISH) The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in situ hybridization kit (Servicebio, Wuhan, China) according to the manufacturer’s protocol. ..

Article Title: ANRIL modulates endothelial senescence and angiogenesis through SASP-driven miR146a regulation in age-related vascular dysfunction.
Article Snippet: Vascular aging, marked by endothelial cell (EC) dysfunction and compromised angiogenesis, is a central driver of age-related ischemic diseases.. Although lncRNAs have emerged as pivotal regulators of endothelial function, their specific roles in endothelial aging remain enigmatic.. In this study, we identify the lncRNA ANRIL as a crucial modulator of endothelial dysfunction during aging.

In Situ Hybridization:

Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway
Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927.

Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier
Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via Fluorescent In Situ Hybridization Kit (Servicebio, Wuhan, China). .. Biotin-labeled probes (Robobio, Guangzhou, China) and an RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA) were used for RNA pull-down in HUVECs (1 × 10 7 ).

Article Title: Circ_0057105 promotes intrahepatic cholangiocarcinoma progression by sponging miR-1290 and regulating the MDM2/P53 pathway.
Article Snippet: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive malignancy with limited treatment options, underscoring the need to identify new regulatory molecules.. Through high-throughput sequencing of five paired ICC and adjacent normal tissues, we identified a significantly upregulated circular RNA, circ_0057105, which is further validated in ICC cell lines and clinical samples.. A correlation between elevated circ_0057105 expression and poor prognosis in ICC patients was observed based on clinical data.

Article Title: Single-nucleus rna sequencing identifies universal camk1d upregulation and dysregulated c-ltmr subtypes as key drivers of paclitaxel-induced neuropathy.
Article Snippet: The snRNA libraries were sequenced on GenoLab M platform (GeneMind Biosciences, Shenzhen, China) via PE150 model (Pavel et al. 2023). .. The fluorescence in situ hybridization (FISH) FISH assay was performed using a Fluorescent In Situ Hybridization Kit (Servicebio, Wuhan, China) following the manufacturer’s protocols. .. The hybridization utilized Camk1d (Camk1d probe sequence 5′- GGG TAA CCA CAG AGC AAG ATA TAG GCG3′) with Alexa Cy3-labeled and 488-labeled probes (Obio Technology, Shanghai, China), respectively.

Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B
Article Snippet: .. The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in situ hybridization kit (Servicebio, Wuhan, China) according to the manufacturer’s protocol. ..

Article Title: LINC01116 modulates EMT process via binding with AGO1 mRNA in oesophageal squamous cell carcinoma.
Article Snippet: For FISH assay, DIGlabeled LINC01116 probe was purchased by Servicebio Company (Wuhan, China). .. Hybridization was performed overnight at 37 ◦C using a fluorescent in situ hybridization kit (Servicebio, China). .. For IF assay, tissues were incubated with antibodies against AGO1 (1:150, Abcam) for 2 h. DAPI was used to stain the nuclei of the cells.

Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B
Article Snippet: .. Fluorescence in situ hybridization (FISH) The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in situ hybridization kit (Servicebio, Wuhan, China) according to the manufacturer’s protocol. ..

Article Title: ANRIL modulates endothelial senescence and angiogenesis through SASP-driven miR146a regulation in age-related vascular dysfunction.
Article Snippet: Vascular aging, marked by endothelial cell (EC) dysfunction and compromised angiogenesis, is a central driver of age-related ischemic diseases.. Although lncRNAs have emerged as pivotal regulators of endothelial function, their specific roles in endothelial aging remain enigmatic.. In this study, we identify the lncRNA ANRIL as a crucial modulator of endothelial dysfunction during aging.

Fluorescence In Situ Hybridization:

Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway
Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927.

Article Title: Single-nucleus rna sequencing identifies universal camk1d upregulation and dysregulated c-ltmr subtypes as key drivers of paclitaxel-induced neuropathy.
Article Snippet: The snRNA libraries were sequenced on GenoLab M platform (GeneMind Biosciences, Shenzhen, China) via PE150 model (Pavel et al. 2023). .. The fluorescence in situ hybridization (FISH) FISH assay was performed using a Fluorescent In Situ Hybridization Kit (Servicebio, Wuhan, China) following the manufacturer’s protocols. .. The hybridization utilized Camk1d (Camk1d probe sequence 5′- GGG TAA CCA CAG AGC AAG ATA TAG GCG3′) with Alexa Cy3-labeled and 488-labeled probes (Obio Technology, Shanghai, China), respectively.

Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B
Article Snippet: .. The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in situ hybridization kit (Servicebio, Wuhan, China) according to the manufacturer’s protocol. ..

Article Title: Circ_0000231 promotes paclitaxel resistance in ovarian cancer by regulating miR-140/RAP1B
Article Snippet: .. Fluorescence in situ hybridization (FISH) The subcellular location of circ_0000231 and miR-140 was detected by FISH assay using a fluorescent in situ hybridization kit (Servicebio, Wuhan, China) according to the manufacturer’s protocol. ..

Article Title: ANRIL modulates endothelial senescence and angiogenesis through SASP-driven miR146a regulation in age-related vascular dysfunction.
Article Snippet: Vascular aging, marked by endothelial cell (EC) dysfunction and compromised angiogenesis, is a central driver of age-related ischemic diseases.. Although lncRNAs have emerged as pivotal regulators of endothelial function, their specific roles in endothelial aging remain enigmatic.. In this study, we identify the lncRNA ANRIL as a crucial modulator of endothelial dysfunction during aging.

Immunocytochemistry:

Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway
Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927.

Article Title: Circ_0057105 promotes intrahepatic cholangiocarcinoma progression by sponging miR-1290 and regulating the MDM2/P53 pathway.
Article Snippet: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive malignancy with limited treatment options, underscoring the need to identify new regulatory molecules.. Through high-throughput sequencing of five paired ICC and adjacent normal tissues, we identified a significantly upregulated circular RNA, circ_0057105, which is further validated in ICC cell lines and clinical samples.. A correlation between elevated circ_0057105 expression and poor prognosis in ICC patients was observed based on clinical data.

Hybridization:

Article Title: Circ_0084927 promotes progression of intrahepatic cholangiocarcinoma by sponging miR-4725-5p to activate the PDPK1/AKT/mTOR signaling pathway
Article Snippet: Background: Numerous studies have indicated that circular RNAs (circRNA) are involved in the regulation of various malignant tumors, including intrahepatic cholangiocarcinoma (ICC).. However, the exact role of circRNA in the progression of ICC and their underlying regulatory mechanisms remain to be further elucidated.. Methods: We investigated the dysregulation of circRNA expression profiles in five pairs of ICC tissues and adjacent normal tissues through RNA sequencing, revealing a significant upregulation of circ_0084927.

Article Title: Circ_0057105 promotes intrahepatic cholangiocarcinoma progression by sponging miR-1290 and regulating the MDM2/P53 pathway.
Article Snippet: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive malignancy with limited treatment options, underscoring the need to identify new regulatory molecules.. Through high-throughput sequencing of five paired ICC and adjacent normal tissues, we identified a significantly upregulated circular RNA, circ_0057105, which is further validated in ICC cell lines and clinical samples.. A correlation between elevated circ_0057105 expression and poor prognosis in ICC patients was observed based on clinical data.

Article Title: LINC01116 modulates EMT process via binding with AGO1 mRNA in oesophageal squamous cell carcinoma.
Article Snippet: For FISH assay, DIGlabeled LINC01116 probe was purchased by Servicebio Company (Wuhan, China). .. Hybridization was performed overnight at 37 ◦C using a fluorescent in situ hybridization kit (Servicebio, China). .. For IF assay, tissues were incubated with antibodies against AGO1 (1:150, Abcam) for 2 h. DAPI was used to stain the nuclei of the cells.

Imaging:

Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier
Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via Fluorescent In Situ Hybridization Kit (Servicebio, Wuhan, China). .. Biotin-labeled probes (Robobio, Guangzhou, China) and an RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA) were used for RNA pull-down in HUVECs (1 × 10 7 ).

Microscopy:

Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier
Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via Fluorescent In Situ Hybridization Kit (Servicebio, Wuhan, China). .. Biotin-labeled probes (Robobio, Guangzhou, China) and an RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA) were used for RNA pull-down in HUVECs (1 × 10 7 ).

Confocal Microscopy:

Article Title: Mechanistic study of the hsa_circ_0074158 binding EIF4A3 impairing sepsis-induced endothelial barrier
Article Snippet: After overnight probe hybridization, nuclei were counterstained with DAPI (Beyotime Biotechnology, Shanghai, China). .. Imaging was performed using an Olympus BX53 microscope and confocal microscopy (Olympus BX53 biological microscope), with signal detection via Fluorescent In Situ Hybridization Kit (Servicebio, Wuhan, China). .. Biotin-labeled probes (Robobio, Guangzhou, China) and an RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA) were used for RNA pull-down in HUVECs (1 × 10 7 ).



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miR-295 is induced in renal tubules in HN mice. HN was induced by PO intraperitoneally and Ad orally administered for 2 weeks. Except for the control group, each group was intraperitoneally administered PO (350 mg/kg per day) and orally administered with Ad (70 mg/kg per day) to induce HN at 8:30 am for 14 consecutive days. Control mice were treated with normal saline. At the end of 21st day, the animals were euthanized. (A) Body weight changes in control and HN mice; (B) serum UA concentrations in each group; (C) serum CRE levels; (D) BUN levels; (E) representative images of HE staining, scale bar: 50 μ m; (F) representative images of Masson staining, scale bar: 50 μ m; (G) quantitative analysis of tubular injury score in different experimental groups; (H) quantification of Masson's trichrome–positive fibrotic area in kidney sections; (I) volcano plot of microRNA expression profiled by microarray in HN. Differentially expressed miRNAs were identified using data obtained from three biological replicates per experimental group ( n =3 mice per group, total six samples). Statistical significance was determined using the criteria of |log 2 fold change| >1.5 and P < 0.05. These thresholds were applied to ensure robust identification of meaningful expression differences. (J) qPCR analysis of miR-295 in mouse kidneys. The level of miR-295 was normalized to the level of U6 (internal loading control) of the same samples to determine the ratio with the ratio of control mice arbitrarily set as 1. (K) In situ <t>hybridization</t> showing miR-295 increase in kidney tissues. Representative images of double immunostaining with miR-295 and proximal tubule marker, LTL, scale bar: 50 μ m. (L) Quantitative analysis of <t>fluorescence</t> intensity. All the values are expressed as mean±SD ( n =6), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Ad, adenine; CRE, creatinine; HE, hematoxylin–eosin; HN, hyperuricemic nephropathy; LTL, Lotus tetragonolobus lectin; PO, potassium oxonate; UA, uric acid
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Expression pattern of circHIPK2 and its characteristics in human chondrocytes. a , b Histological analysis, preoperative Kellgren-Lawrence and OARSI grading of sequencing cartilage sample (n = 3 per group). c Heatmap of circRNA expression in OA and NA chondrocytes. d RT-qPCR analysis of circHIPK2, circGOSR2, circREPS1, circMTUS1, circNUP54, circSLC8A1, circADAMTS6, circFN1, circTNFRSF21 and circAPBB2 expression in NA and OA chondrocytes ( n = 6 per group). e Representative images of circHIPK2 expression in NA and OA chondrocytes via FISH staining, γH2AX staining, SA-β gal staining. f CircHIPK2 expression pattern in chondrocytes treated with IL-1β, Etoposide and TNF-α, assessed via RT-qPCR ( n = 6 per group). g <t>RNA</t> fluorescence in situ <t>hybridization</t> (FISH) staining of circHIPK2 in aging mice and DMM mice. h Schematic illustration depicting HIPK2 exons 2 circularization to form circHIPK2. The presence of circHIPK2 was validated by RT-qPCR, followed by Sanger sequencing, red box represents head-to-tail circHIPK2 splicing sites. i RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with or without RNase R ( n = 6 per group). j RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with actinomycin D for different durations ( n = 3 per group). k RT-qPCR analysis of circHIPK2 and HIPK2 expression in NA and OA chondrocytes ( n = 10 per group). l Representative images of FISH staining for circHIPK2 localization. m RT-qPCR analysis of circHIPK2 expression in the nuclear and cytoplasmic fractions. GAPDH served as control. ** P < 0.01, *** P < 0.001
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Expression pattern of circHIPK2 and its characteristics in human chondrocytes. a , b Histological analysis, preoperative Kellgren-Lawrence and OARSI grading of sequencing cartilage sample (n = 3 per group). c Heatmap of circRNA expression in OA and NA chondrocytes. d RT-qPCR analysis of circHIPK2, circGOSR2, circREPS1, circMTUS1, circNUP54, circSLC8A1, circADAMTS6, circFN1, circTNFRSF21 and circAPBB2 expression in NA and OA chondrocytes ( n = 6 per group). e Representative images of circHIPK2 expression in NA and OA chondrocytes via FISH staining, γH2AX staining, SA-β gal staining. f CircHIPK2 expression pattern in chondrocytes treated with IL-1β, Etoposide and TNF-α, assessed via RT-qPCR ( n = 6 per group). g <t>RNA</t> fluorescence in situ <t>hybridization</t> (FISH) staining of circHIPK2 in aging mice and DMM mice. h Schematic illustration depicting HIPK2 exons 2 circularization to form circHIPK2. The presence of circHIPK2 was validated by RT-qPCR, followed by Sanger sequencing, red box represents head-to-tail circHIPK2 splicing sites. i RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with or without RNase R ( n = 6 per group). j RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with actinomycin D for different durations ( n = 3 per group). k RT-qPCR analysis of circHIPK2 and HIPK2 expression in NA and OA chondrocytes ( n = 10 per group). l Representative images of FISH staining for circHIPK2 localization. m RT-qPCR analysis of circHIPK2 expression in the nuclear and cytoplasmic fractions. GAPDH served as control. ** P < 0.01, *** P < 0.001
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Expression pattern of circHIPK2 and its characteristics in human chondrocytes. a , b Histological analysis, preoperative Kellgren-Lawrence and OARSI grading of sequencing cartilage sample (n = 3 per group). c Heatmap of circRNA expression in OA and NA chondrocytes. d RT-qPCR analysis of circHIPK2, circGOSR2, circREPS1, circMTUS1, circNUP54, circSLC8A1, circADAMTS6, circFN1, circTNFRSF21 and circAPBB2 expression in NA and OA chondrocytes ( n = 6 per group). e Representative images of circHIPK2 expression in NA and OA chondrocytes via FISH staining, γH2AX staining, SA-β gal staining. f CircHIPK2 expression pattern in chondrocytes treated with IL-1β, Etoposide and TNF-α, assessed via RT-qPCR ( n = 6 per group). g <t>RNA</t> fluorescence in situ <t>hybridization</t> (FISH) staining of circHIPK2 in aging mice and DMM mice. h Schematic illustration depicting HIPK2 exons 2 circularization to form circHIPK2. The presence of circHIPK2 was validated by RT-qPCR, followed by Sanger sequencing, red box represents head-to-tail circHIPK2 splicing sites. i RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with or without RNase R ( n = 6 per group). j RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with actinomycin D for different durations ( n = 3 per group). k RT-qPCR analysis of circHIPK2 and HIPK2 expression in NA and OA chondrocytes ( n = 10 per group). l Representative images of FISH staining for circHIPK2 localization. m RT-qPCR analysis of circHIPK2 expression in the nuclear and cytoplasmic fractions. GAPDH served as control. ** P < 0.01, *** P < 0.001
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Expression pattern of circHIPK2 and its characteristics in human chondrocytes. a , b Histological analysis, preoperative Kellgren-Lawrence and OARSI grading of sequencing cartilage sample (n = 3 per group). c Heatmap of circRNA expression in OA and NA chondrocytes. d RT-qPCR analysis of circHIPK2, circGOSR2, circREPS1, circMTUS1, circNUP54, circSLC8A1, circADAMTS6, circFN1, circTNFRSF21 and circAPBB2 expression in NA and OA chondrocytes ( n = 6 per group). e Representative images of circHIPK2 expression in NA and OA chondrocytes via FISH staining, γH2AX staining, SA-β gal staining. f CircHIPK2 expression pattern in chondrocytes treated with IL-1β, Etoposide and TNF-α, assessed via RT-qPCR ( n = 6 per group). g <t>RNA</t> fluorescence in situ <t>hybridization</t> (FISH) staining of circHIPK2 in aging mice and DMM mice. h Schematic illustration depicting HIPK2 exons 2 circularization to form circHIPK2. The presence of circHIPK2 was validated by RT-qPCR, followed by Sanger sequencing, red box represents head-to-tail circHIPK2 splicing sites. i RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with or without RNase R ( n = 6 per group). j RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with actinomycin D for different durations ( n = 3 per group). k RT-qPCR analysis of circHIPK2 and HIPK2 expression in NA and OA chondrocytes ( n = 10 per group). l Representative images of FISH staining for circHIPK2 localization. m RT-qPCR analysis of circHIPK2 expression in the nuclear and cytoplasmic fractions. GAPDH served as control. ** P < 0.01, *** P < 0.001
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miR-295 is induced in renal tubules in HN mice. HN was induced by PO intraperitoneally and Ad orally administered for 2 weeks. Except for the control group, each group was intraperitoneally administered PO (350 mg/kg per day) and orally administered with Ad (70 mg/kg per day) to induce HN at 8:30 am for 14 consecutive days. Control mice were treated with normal saline. At the end of 21st day, the animals were euthanized. (A) Body weight changes in control and HN mice; (B) serum UA concentrations in each group; (C) serum CRE levels; (D) BUN levels; (E) representative images of HE staining, scale bar: 50 μ m; (F) representative images of Masson staining, scale bar: 50 μ m; (G) quantitative analysis of tubular injury score in different experimental groups; (H) quantification of Masson's trichrome–positive fibrotic area in kidney sections; (I) volcano plot of microRNA expression profiled by microarray in HN. Differentially expressed miRNAs were identified using data obtained from three biological replicates per experimental group ( n =3 mice per group, total six samples). Statistical significance was determined using the criteria of |log 2 fold change| >1.5 and P < 0.05. These thresholds were applied to ensure robust identification of meaningful expression differences. (J) qPCR analysis of miR-295 in mouse kidneys. The level of miR-295 was normalized to the level of U6 (internal loading control) of the same samples to determine the ratio with the ratio of control mice arbitrarily set as 1. (K) In situ hybridization showing miR-295 increase in kidney tissues. Representative images of double immunostaining with miR-295 and proximal tubule marker, LTL, scale bar: 50 μ m. (L) Quantitative analysis of fluorescence intensity. All the values are expressed as mean±SD ( n =6), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Ad, adenine; CRE, creatinine; HE, hematoxylin–eosin; HN, hyperuricemic nephropathy; LTL, Lotus tetragonolobus lectin; PO, potassium oxonate; UA, uric acid

Journal: Kidney360

Article Title: The Positive Feedback Loop of Hypoxia-Inducible Factor-1 α /miR-295/Factor Inhibiting Hypoxia-Inducible Factor-1 in Hyperuricemic Nephropathy

doi: 10.34067/KID.0000001069

Figure Lengend Snippet: miR-295 is induced in renal tubules in HN mice. HN was induced by PO intraperitoneally and Ad orally administered for 2 weeks. Except for the control group, each group was intraperitoneally administered PO (350 mg/kg per day) and orally administered with Ad (70 mg/kg per day) to induce HN at 8:30 am for 14 consecutive days. Control mice were treated with normal saline. At the end of 21st day, the animals were euthanized. (A) Body weight changes in control and HN mice; (B) serum UA concentrations in each group; (C) serum CRE levels; (D) BUN levels; (E) representative images of HE staining, scale bar: 50 μ m; (F) representative images of Masson staining, scale bar: 50 μ m; (G) quantitative analysis of tubular injury score in different experimental groups; (H) quantification of Masson's trichrome–positive fibrotic area in kidney sections; (I) volcano plot of microRNA expression profiled by microarray in HN. Differentially expressed miRNAs were identified using data obtained from three biological replicates per experimental group ( n =3 mice per group, total six samples). Statistical significance was determined using the criteria of |log 2 fold change| >1.5 and P < 0.05. These thresholds were applied to ensure robust identification of meaningful expression differences. (J) qPCR analysis of miR-295 in mouse kidneys. The level of miR-295 was normalized to the level of U6 (internal loading control) of the same samples to determine the ratio with the ratio of control mice arbitrarily set as 1. (K) In situ hybridization showing miR-295 increase in kidney tissues. Representative images of double immunostaining with miR-295 and proximal tubule marker, LTL, scale bar: 50 μ m. (L) Quantitative analysis of fluorescence intensity. All the values are expressed as mean±SD ( n =6), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Ad, adenine; CRE, creatinine; HE, hematoxylin–eosin; HN, hyperuricemic nephropathy; LTL, Lotus tetragonolobus lectin; PO, potassium oxonate; UA, uric acid

Article Snippet: The Digoxigenin-labeled mmu-miR-295 locked nucleic acid (LNA) probe and Fluorescence in situ hybridization (FISH) Kit were from Servicebio (Wuhan, China). miR-295 mimic, anti-miR-295 LNA, and FIH-1 siRNA were from Ruibo (Guangzhou, China).

Techniques: Control, Saline, Staining, Expressing, Microarray, In Situ Hybridization, Double Immunostaining, Marker, Fluorescence

Expression pattern of circHIPK2 and its characteristics in human chondrocytes. a , b Histological analysis, preoperative Kellgren-Lawrence and OARSI grading of sequencing cartilage sample (n = 3 per group). c Heatmap of circRNA expression in OA and NA chondrocytes. d RT-qPCR analysis of circHIPK2, circGOSR2, circREPS1, circMTUS1, circNUP54, circSLC8A1, circADAMTS6, circFN1, circTNFRSF21 and circAPBB2 expression in NA and OA chondrocytes ( n = 6 per group). e Representative images of circHIPK2 expression in NA and OA chondrocytes via FISH staining, γH2AX staining, SA-β gal staining. f CircHIPK2 expression pattern in chondrocytes treated with IL-1β, Etoposide and TNF-α, assessed via RT-qPCR ( n = 6 per group). g RNA fluorescence in situ hybridization (FISH) staining of circHIPK2 in aging mice and DMM mice. h Schematic illustration depicting HIPK2 exons 2 circularization to form circHIPK2. The presence of circHIPK2 was validated by RT-qPCR, followed by Sanger sequencing, red box represents head-to-tail circHIPK2 splicing sites. i RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with or without RNase R ( n = 6 per group). j RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with actinomycin D for different durations ( n = 3 per group). k RT-qPCR analysis of circHIPK2 and HIPK2 expression in NA and OA chondrocytes ( n = 10 per group). l Representative images of FISH staining for circHIPK2 localization. m RT-qPCR analysis of circHIPK2 expression in the nuclear and cytoplasmic fractions. GAPDH served as control. ** P < 0.01, *** P < 0.001

Journal: Molecular Biomedicine

Article Title: YTH N6-methyladenosine RNA binding protein 2 mediated m 6 A modification of circHIPK2 promotes cellular senescence and osteoarthritis progression by inhibiting autophagy

doi: 10.1186/s43556-026-00441-4

Figure Lengend Snippet: Expression pattern of circHIPK2 and its characteristics in human chondrocytes. a , b Histological analysis, preoperative Kellgren-Lawrence and OARSI grading of sequencing cartilage sample (n = 3 per group). c Heatmap of circRNA expression in OA and NA chondrocytes. d RT-qPCR analysis of circHIPK2, circGOSR2, circREPS1, circMTUS1, circNUP54, circSLC8A1, circADAMTS6, circFN1, circTNFRSF21 and circAPBB2 expression in NA and OA chondrocytes ( n = 6 per group). e Representative images of circHIPK2 expression in NA and OA chondrocytes via FISH staining, γH2AX staining, SA-β gal staining. f CircHIPK2 expression pattern in chondrocytes treated with IL-1β, Etoposide and TNF-α, assessed via RT-qPCR ( n = 6 per group). g RNA fluorescence in situ hybridization (FISH) staining of circHIPK2 in aging mice and DMM mice. h Schematic illustration depicting HIPK2 exons 2 circularization to form circHIPK2. The presence of circHIPK2 was validated by RT-qPCR, followed by Sanger sequencing, red box represents head-to-tail circHIPK2 splicing sites. i RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with or without RNase R ( n = 6 per group). j RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with actinomycin D for different durations ( n = 3 per group). k RT-qPCR analysis of circHIPK2 and HIPK2 expression in NA and OA chondrocytes ( n = 10 per group). l Representative images of FISH staining for circHIPK2 localization. m RT-qPCR analysis of circHIPK2 expression in the nuclear and cytoplasmic fractions. GAPDH served as control. ** P < 0.01, *** P < 0.001

Article Snippet: A RNA fluorescent in situ hybridization kit (cat. no. GF-003, Servicebio, China) was used to detect circHIPK2 expression in chondrocytes and knee joint sections.

Techniques: Expressing, Sequencing, Quantitative RT-PCR, Staining, Fluorescence, In Situ Hybridization, Control